FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329130
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT128013.889344635858172TruSeq Adapter, Index 9 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA16660.5061829672166013No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7200.21875854525567404No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT6050.18381794427733722No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT5770.17531066751739433TruSeq Adapter, Index 9 (97% over 36bp)
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG4720.14340837966760855No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4470.13581259684623095No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGGAT3750.11393674232066356TruSeq Adapter, Index 9 (97% over 36bp)
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACG3470.10542946556072069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)