FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305982
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT175695.74184102332817TruSeq Adapter, Index 9 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11800.3856435999503239No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT6930.22648391081828345TruSeq Adapter, Index 9 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA5460.17844186912955665No Hit
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA5000.16340830506369655No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4360.14249204201554339No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG4010.13105346066108464No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3940.1287657443901929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)