FastQCFastQC Report
Wed 8 May 2024
EGAF00006128511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305982
Total Bases43.7 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11910.3892385826617252No Hit
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA4740.15491107320038433No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA4530.14804792438770908No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4300.14053114235477904No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3800.12419031184840938No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG3460.11307854710407803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)