FastQCFastQC Report
Wed 8 May 2024
EGAF00006128520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406338
Total Bases56.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT171944.231452633029646TruSeq Adapter, Index 8 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24900.6127903371085155No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG7260.17866898985573587No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA6920.17030157159802922No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT5820.14323051252897834No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA5760.1417539093070301No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTACTTCAAGTCACAAAGGGA5390.13264818943834936No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4750.11689775507090155No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT4300.10582323090628984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)