FastQCFastQC Report
Wed 8 May 2024
EGAF00006128521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406338
Total Bases56.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25500.6275563693279979No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA6730.16562566139519316No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG6620.16291855548828807No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT5730.141015607696056No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTACTTCAAGTCACAAAGGGA5560.13683189856720268No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA5270.1296949829944529No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4930.12132756473674626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)