FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406338
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25500.6275563693279979No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA6530.16070365065536574No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG6470.1592270474334175No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT5600.13781630071516815No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTACTTCAAGTCACAAAGGGA5430.13363259158631483No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA5140.12649567601356507No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4830.11886655936683255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)