FastQCFastQC Report
Wed 8 May 2024
EGAF00006128524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398708
Total Bases55.5 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT128663.2269229611645613TruSeq Adapter, Index 2 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28840.7233363764960823No Hit
GCATTTCCTTGCTTGCTCACTTCAGTCCCTCTAGCCTTCTTTCTGTCCCT8130.20390862485829228No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACATCAGCCCCTCTA8040.2016513338082005No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT7150.17932923342395937No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT5390.13518665288883092No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA5070.12716072915517118No Hit
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA4410.11060726145449802No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG4160.10433700853757638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)