FastQCFastQC Report
Wed 8 May 2024
EGAF00006128528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222552
Total Bases32.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT104204.682051835076746TruSeq Adapter, Index 2 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCTTTCTA5030.22601459434199647No Hit
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA3720.1671519465113771No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACGTCAGCCCCTCTA3250.1460332866026816No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTT2690.12087062798806572No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2430.10918796505985118No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT2260.10154930083755707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)