FastQCFastQC Report
Wed 8 May 2024
EGAF00006128534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356188
Total Bases50 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATCTCGTAT138303.8827810032903978TruSeq Adapter, Index 7 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20500.5755387604298854No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT5850.16423910968365021No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA5360.15048232955630175No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4760.13363729266567095No Hit
GGCCTGGAGAGGCTCTGCGACCCGCTTAGGACCACAGAACTCGGTACTAG4060.11398474962660168No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA4020.1128617471672263No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT3950.11089649286331936No Hit
GCGTGACAGCCACTGTAGGACTTTGATCTCAGGGGGACAAGCTGACACAG3610.10135097195862859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)