FastQCFastQC Report
Wed 8 May 2024
EGAF00006128540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272551
Total Bases39.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATCTCGTAT106903.922201716376018TruSeq Adapter, Index 18 (97% over 39bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA9140.33535008126919363No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA7140.26196931950350577No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATATCGTAT5250.1926244996349307TruSeq Adapter, Index 18 (97% over 39bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4900.17978286632593532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)