FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006128621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162425947
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6140330.37803873786249187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA2238090.1377914084133368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT16579950.0125.43021
AGCAGTG18511850.0112.332112
GCAGTGG22653650.091.797453
CGACGCT659300.086.109516
CAGTGGT24897500.083.529254
AGTGGTA25085200.082.885595
CACGACG712400.080.2975854
ACGACGC732500.078.221985
GTGGTAT27128000.076.626076
TGGTATC27844800.074.6599667
GGTATCA28050450.074.221188
ACGCTCT766250.073.705458
GTATCAA28560350.072.9147349
GACGCTC828950.068.252137
CGCTCTT977650.057.8268289
ACACGAC1518500.037.8231163
TACACGA1535750.037.407652
CTACACG1724000.033.4900251
AACGCAG28653300.024.24640510-14
AGAGTAC29089300.023.767815-19
Produced by FastQC (version 0.11.5)