FastQCFastQC Report
Tue 26 Apr 2022
EGAF00006128633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119036231
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5554200.4665974345239476No Hit
AAGCAGTGGTATCAACGCAGAGAGTAATGCTGAACTTAAGGGTTTAGATG2069470.1738521106233614Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTCCGCCAGACCGCCGC1519700.12766701257535615Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)