FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006128635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117341708
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4943440.4212858398140924No Hit
AAGCAGTGGTATCAACGCAGAGAGTAATGCTGAACTTAAGGGTTTAGATG1881220.16031980717376296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT11055600.0122.435821
AGCAGTG11816950.0114.63582
GTGGTAT13191800.0102.636696
AGTGGTA13275950.0102.028875
GCAGTGG13339700.0101.566533
GTATCAA13811950.098.0749059
GGTATCA14041300.096.4784558
TGGTATC14278400.094.888997
CAGTGGT14315250.094.714494
ACGCTCT273700.081.5446858
CACGACG312850.072.777364
CGACGCT311450.072.181896
GACGCTC318500.070.3922357
ACGACGC414800.054.3704835
CGCTCTT529850.042.409279
AACGCAG13312550.027.2363310-14
TACATGG12988150.026.39471820-24
TCAACGC12987000.026.3580510-14
CAACGCA13696500.026.0304410-14
AGAGTAC13479200.025.90653615-19
Produced by FastQC (version 0.11.5)