FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006128647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142951145
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7215800.5047738512342801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT21097100.0133.649141
AGCAGTG24153850.0116.8492362
GCAGTGG28710300.098.3132863
CAGTGGT31420500.089.82734
AGTGGTA31961400.088.266465
GTGGTAT35103550.080.36226
TGGTATC35597700.079.2417147
GGTATCA36201250.077.923978
GTATCAA36539850.077.209599
CGACGCT503750.070.215986
ACGACGC573600.061.8912245
GACGCTC571700.061.8832367
CACGACG602750.059.0527234
CGCTCTT745950.047.504829
ACACGAC1008900.035.2303623
TACACGA1034650.034.416232
CTACACG1234850.028.988061
AACGCAG36881050.024.41573710-14
AGAGTAC37114700.024.1009115-19
ACATGGG36011050.023.68623720-24
Produced by FastQC (version 0.11.5)