FastQCFastQC Report
Sun 19 May 2024
EGAF00006128667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185269837
Total Bases22.2 Gbp
Sequences flagged as poor quality0
Sequence length120
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA6278410.33887923159342986Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3543970.1912869389527233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2587350.13965306182031131Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2138650.11543433268093176No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)