FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006128673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110903717
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5816640.5244765601499182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT11274950.0127.512671
AGCAGTG13208950.0108.953692
GCAGTGG16480350.087.3083953
CACGACG400450.079.3169864
CAGTGGT18355500.078.3989264
AGTGGTA18738250.076.7599645
CGACGCT429450.073.861326
GTGGTAT21695250.066.285076
TGGTATC22150400.064.934187
GGTATCA22914400.062.7630278
ACGACGC508400.062.4474265
GTATCAA23150050.062.128619
GACGCTC512700.061.8678937
ACGCTCT598100.052.8310248
CGCTCTT698900.045.4159749
ACACGAC1058600.030.186643
TACACGA1075000.029.6996382
CTACACG1194950.026.8917871
ATCAACG23080900.022.7127369
AACGCAG23346000.022.2812210-14
Produced by FastQC (version 0.11.5)