FastQCFastQC Report
Fri 22 Apr 2022
EGAF00006128677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283364285
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14398080.5081120226566309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT17715700.0117.248541
AGCAGTG20572800.0100.984322
CACGACG800300.092.4774864
GCAGTGG24653900.084.263153
ACGACGC880150.084.1782155
CGACGCT955950.077.4433066
AGTGGTA26898000.077.179715
CAGTGGT27598000.075.317474
ACGCTCT1056300.069.854878
GTGGTAT31108650.066.708266
TGGTATC32248400.064.376357
GGTATCA32721400.063.421618
GTATCAA33161050.062.591229
GACGCTC1380450.053.6760147
CGCTCTT1412400.052.5331959
ACACGAC2109200.035.3348353
TACACGA2177550.034.209212
CTACACG2599450.028.8922861
AACGCAG33312850.022.10537110-14
AGAGTAC33741600.021.62000515-19
Produced by FastQC (version 0.11.5)