FastQCFastQC Report
Fri 22 Apr 2022
EGAF00006128681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150637653
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7435160.4935791186284614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT11206950.0119.788171
AGCAGTG12615200.0106.486552
CACGACG491200.088.020024
GCAGTGG15784700.085.125083
ACGACGC555600.077.766335
ACGCTCT559800.076.849128
AGTGGTA17563000.076.457295
CAGTGGT17732950.075.818524
CGACGCT571900.075.3989646
GTGGTAT20602300.065.156896
GACGCTC681450.063.394217
TGGTATC21464550.062.577617
GGTATCA22044100.060.901128
GTATCAA22199300.060.479469
CGCTCTT810350.053.3368729
ACACGAC1377850.031.5514243
TACACGA1383350.031.4051572
CTACACG1655800.026.4941041
AACGCAG22486900.021.61111310-14
AGAGTAC22746200.021.15986615-19
Produced by FastQC (version 0.11.5)