FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006128701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85492697
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2722020.3183921078077581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7487500.0124.601741
AGCAGTG8925250.0104.6517942
GCAGTGG11073900.084.38313
CACGACG354500.079.7476964
AGTGGTA11768850.079.346495
CAGTGGT11939600.078.27874
GACGCTC363250.077.5705647
CGACGCT371350.075.780826
GTGGTAT12826400.072.779656
ACGACGC397100.071.0115055
TGGTATC13273400.070.3759167
GGTATCA13392000.069.731718
GTATCAA13467450.069.347659
CGCTCTT486300.057.9725659
ACGCTCT490950.057.3350458
TACACGA671850.041.929082
ACACGAC678950.041.606863
CTACACG838550.034.1420971
ATCAACG13175250.025.1733269
AACGCAG13433850.024.37588310-14
Produced by FastQC (version 0.11.5)