FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006128721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126083688
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5098360.4043631718640717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT1980290.15706155422737952No Hit
AAGCAGTGGTATCAACGCAGAGAGTAATGCTGAACTTAAGGGTTTAGATG1459250.1157366209021424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTCCGCCAGACCGCCGC1275630.1011732778628747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1274930.10111775918229803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT41196000.0138.784241
AGCAGTG44714650.0127.9622962
GCAGTGG48475300.0118.029433
AGTGGTA49337350.0115.9637455
GTGGTAT49488750.0115.571416
CAGTGGT49656100.0115.220284
GGTATCA49824550.0114.782138
TGGTATC49897250.0114.6119847
GTATCAA50244650.0113.842369
ACGCTCT340700.079.918448
GACGCTC365850.074.699517
CACGACG371800.073.7940144
CGACGCT376450.072.500516
ACGACGC470150.058.081785
CGCTCTT586300.046.4530459
AACGCAG49787950.028.10274310-14
CAACGCA49725800.027.85915210-14
TACATGG49420800.027.6416120-24
AGAGTAC50003850.027.6128915-19
ACATGGG49075650.027.5684420-24
Produced by FastQC (version 0.11.5)