FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006128725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156262080
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5921810.378966541338756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT10466500.0114.74461
AGCAGTG11257450.0106.6487352
CACGACG480900.097.676854
ACGCTCT517500.089.502618
GCAGTGG13578950.088.439063
ACGACGC540950.086.7540365
CGACGCT550700.084.5905156
AGTGGTA14277350.084.0730745
CAGTGGT15094700.079.679044
GTGGTAT15452900.077.622876
GTATCAA16534050.072.67919
GGTATCA16702150.071.974358
TGGTATC16805350.071.451387
GACGCTC661100.070.192117
CGCTCTT840700.055.2739339
ACACGAC1825750.025.952733
TACACGA1832400.025.8624742
AACGCAG16284050.023.92281510-14
AGAGTAC16656850.023.1731715-19
CCGATCT449950.022.26487415-19
Produced by FastQC (version 0.11.5)