FastQCFastQC Report
Sat 28 May 2022
EGAF00006130652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9166747
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCG1709961.8653945614512977TruSeq Adapter, Index 7 (97% over 35bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCG353920.38609116189199943TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA116440.12702434135031762TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)