FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006130653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9166747
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGGGGG1213751.3240793053413604Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGGTGG210220.22932889933582765Illumina Single End PCR Primer 1 (96% over 33bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGGGGT174360.19020924216627774Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)