FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006130756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4785111
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCG93325419.50328842946381TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA51120.10683137757932888TruSeq Adapter, Index 7 (97% over 35bp)
CGAGATCGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGT50250.10501323793742716TruSeq Adapter, Index 9 (96% over 31bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTAGCTCG47890.10008127293180868TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)