FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34785960
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG442550.12722086726943857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT256650.021.0309771
GGGCGAT44600.017.0682561
TCAATCG32000.016.9073583
GGCGATC47900.016.8272081
GCGCGTG344550.016.2283692
CGCGTGC377000.014.4516213
CAATCGC34700.014.4018734
GCGTGCC395500.013.6030684
CGATCTG65750.012.3168083
ACGTCGT20350.012.0259335
TCACGAA70000.012.01971615
CGTGCCT453950.011.9438835
CGTATCA57000.011.93384265
CGTAGGG29550.011.918653564
TGGCGCG195100.011.8873173
GCCACGA93250.011.76261619
CGCCGTA57650.011.73861462
CGCGGTG130900.011.7361851
TGCGACA79700.011.6790246
ACGTAGG32250.011.67006163
Produced by FastQC (version 0.11.5)