FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32352927
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT725590.2242733709997862No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCA425760.13159860311866065No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTT418300.12929278392647442No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG335640.1037433181857085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG119500.025.7518924
TACGTTC122750.024.9576127
AGCATCG73950.024.86588539
CCTACGT124150.024.64842225
CTACGTT128600.023.87594426
ACCGCGT47600.023.1203966
CCGCGTT48350.022.6904017
CGTCTGA107300.022.2493816
GACTACC147250.021.1799719
ACACGTC117900.020.39531913
ACGTCTG121050.019.95008515
GCATCGG72150.019.70043240
ATCGCGT9500.019.61039742
ACCCTAC159850.019.5752323
ACGTTCT159700.019.5503828
GACCGCG57200.019.5416185
ACTACCC159300.019.53452920
AGACCGC57000.019.4891344
GTCGATT23750.019.3295231
TACCCTA160900.019.29739422
Produced by FastQC (version 0.11.5)