FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31212795
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT373920.11979702554673491No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCA339620.10880794238388455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATCG82400.030.27090839
CGTCTGA118750.027.860916
GCATCGG69050.026.83026540
ACACGTC127900.026.13746313
ACCGCGT54500.025.700396
ACGTCTG131300.025.46063615
CCCTACG102750.025.28273424
TACGTTC107300.024.40354527
CCTACGT107300.024.37139325
CATCGGG75100.024.34742541
CCGCGTT58900.023.7804997
CTACGTT111400.023.66023426
CACGTCT144900.023.07095514
GATCGGA144850.022.638071
AGACCGC63050.022.3793984
ATCGCGT10650.022.35204742
ATCGGGT26000.022.0268642
GACCGCG64950.021.7778435
ATCGGAA154950.021.3967382
CACACGT158700.021.23869312
Produced by FastQC (version 0.11.5)