FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31212795
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT140200.018.5016171
GCGCGTG194950.013.9263542
GGCGATC35250.013.1928131
CGCGTGC205250.012.7273163
TCACGAA61650.012.529428515
GGGCGAT38450.012.005251
TCAATCG22700.011.690033
GCGTGCC221350.011.522694
GGGAGCG177350.011.09795968
AGTGCGT68650.010.6529530
TCCGGAT74150.010.56660657
CGAAGGT75200.010.50374318
CGGATGT76100.010.29627559
GTAGCGT45550.010.23372369
GCCACGA68950.010.20533519
CACTCCG76200.010.1915654
ACGAAGG77250.010.13459517
TGGCGCG123400.010.0260553
CGTGCCT260200.09.94895655
CAATCGC27050.09.8112674
Produced by FastQC (version 0.11.5)