FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29987142
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT495280.1651641226763124No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCA358340.11949788345951742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATCG188050.034.7839939
GCATCGG148300.033.22013540
CATCGGG153150.031.40225841
ATCGCGT21500.030.96948242
CGTCTGA284600.030.23243116
ACGTCTG294700.029.21971315
ATCGGGT50100.028.92188842
CGCGTAT11600.028.55160544
ACACGTC303200.028.54847713
CACGTCT310600.027.76834914
TCGCGTA12050.027.19900943
CCCTACG109200.026.82233224
ACAATCC327450.026.47662432
ATCGCGG31900.026.0640242
CACACGT334200.026.01390612
CATCGCG58250.026.0006241
GCACACG338800.025.7523511
GTCACAA339800.025.60549729
TACGTTC115350.025.42218227
CCTACGT115350.025.30254725
Produced by FastQC (version 0.11.5)