FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29987142
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG330960.1103673034262485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT160100.019.8162121
TCAATCG28600.016.2680453
GCGCGTG223750.014.9513462
GGCGATC35500.014.16696551
GGGCGAT32600.013.9478951
CGCGTGC235950.013.6133053
CAATCGC33550.013.1501984
GCGTGCC250050.012.6265124
CGTAGGG24350.012.61999764
GCGACAT55350.012.3334397
TGCGACA55500.012.0509146
CGCCGTA55650.011.9119262
CGTATCA55700.011.90196365
CCGTATC57100.011.549561564
GGGAGCG219750.011.5487868
ACGTCGT16400.011.3491864
ACGTAGG28750.011.168694563
GCCGTAT59400.011.16020463
CGTGCCT288650.011.0224355
CGGGAGC237850.010.989511567
Produced by FastQC (version 0.11.5)