FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35692904
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC51800.021.7458081
GGGCGAT39400.021.4203911
GGCGCGT136900.020.3313161
CGATCTG67600.017.6395683
TGCGACA102700.016.046086
GCGCGTG178900.015.8882492
GCGACAT105300.015.748837
CGCGTGC208300.014.0898981
GCTGCGA121400.013.9687044
CGACATC121450.013.9104478
CTGCGAC121500.013.9019345
GCGTGCC219050.013.0075252
AGTGCGT74050.011.7857930
GCGATCT103200.011.6847882
CGTGCCT266700.011.0355363
TTGATCG152850.010.92286225
GCCACGA80900.010.87208419
TCAATCG29350.010.6854263
TGATCGC159050.010.67042826
TGGCGCG121200.010.3504043
Produced by FastQC (version 0.11.5)