FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29722040
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG535960.18032409619258974No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGT373720.12573834097524933No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGT363670.12235701183364264No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAG310960.10462269749990244No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT307770.10354941989177055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT268550.020.268541
GGGCGAT42650.019.708851
GGCGATC49450.017.1379681
GCGCGTG355750.015.6999172
TCAATCG30450.015.619433
CGCGTGC387000.014.0886343
CGCGGTG109500.013.3081221
GCGTGCC405900.013.2812174
ACGTCGT18000.013.213865
GGGAGCG317100.013.16474968
CGATCTG66800.013.1564013
TGGCGCG195200.012.8712623
GCCACGA92200.012.79446319
TGCGACA82500.012.7873096
CAATCGC34650.012.53393654
GCGACAT85650.012.5188927
CGGGAGC345600.012.26902767
GGAGCGG347550.012.09059769
CGTGCCT457300.011.8873125
GGTGGCG222350.011.4343041
Produced by FastQC (version 0.11.5)