FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31249859
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG507070.16226313213125218No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGT345810.11065969929656323No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGT326710.10454767171909479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT238600.020.876741
GGCGATC45600.019.037091
GGGCGAT40100.018.4696621
GCGCGTG319300.016.1320112
TCAATCG25150.014.7998713
CGCGTGC355000.014.2129713
GCCACGA104100.013.68435919
CGATCTG64050.013.4521923
GCGTGCC372300.013.4153224
CGCGGTG109500.013.2758761
TGCGACA79850.013.0391416
GCGACAT81400.012.7916117
GGGAGCG298450.012.49462468
ACGAGCT117550.012.0606922
AGTGCGT118250.012.04819730
CGTGCCT420350.011.9565245
TGGCGCG181950.011.9522453
CACGAGC124700.011.56240521
CGGGAGC330900.011.41552367
GGAGCGG330300.011.40466169
Produced by FastQC (version 0.11.5)