FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28525093
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC52350.022.110131
GGCGCGT163100.019.3257271
GGGCGAT51650.017.540991
CGATCTG70750.016.9523683
GCGCGTG213550.014.9075892
TGCGACA98550.014.4132856
GCGACAT99850.014.2609617
CGCGTGC228550.013.8583593
GCGTGCC236750.013.423314
CGACATC113450.012.642918
CTGCGAC114100.012.5075955
GCTGCGA114950.012.3239974
CGGTCGG71750.011.3372794
CGTAGGG20950.011.20724964
AGTGCGT71950.010.78775830
CGGTTCG56150.010.75124128
CGGGGTT93750.010.729521
CGTGCCT300200.010.7019855
GCCACGA73000.010.67892819
CGCACGA16550.010.63022628
Produced by FastQC (version 0.11.5)