FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30268330
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT423420.13988878805008403No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCA363430.12006939266223146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATCG97950.034.58759739
CGTCTGA140600.031.67769216
GCATCGG81150.031.20476240
ACGTCTG152450.029.28326415
CATCGGG86150.028.87309541
ACACGTC156200.028.60232713
CACGTCT164250.027.24251714
ACCGCGT54400.026.381966
CCGCGTT56800.025.3887047
GATCGGA174550.025.3301121
ATCGCGT13100.025.28210842
CCCTACG113650.025.1346924
ATCGGGT33250.024.79816842
CCTACGT116500.024.57903325
ATCGGAA182850.024.3959872
CACACGT184950.024.30539712
GCACACG186400.024.22737511
TACGTTC117800.024.16135627
CTACGTT121250.023.70150426
ATCGCGG18800.023.48905442
Produced by FastQC (version 0.11.5)