FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006130787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69843163
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG809460.1158968129779575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG80700.021.8226183
GGCGCGT539900.018.879361
CAATCGC93450.018.4786764
GGCGATC103650.015.8527531
GGGCGAT97700.015.05528451
AATCGCC128100.014.6386335
GCGCGTG773750.013.8613942
CGCGTGC777800.013.3369983
GCCACGA220800.013.309648519
GCGTGCC786050.012.8872984
TCACGAA138700.012.38117515
ACGTCGT50450.012.1621285
ACGAGCT249700.011.71450522
AGTGCGT250850.011.66131130
ATCAATC174150.011.6525312
CGTGCCT903150.011.339495
ATCGCCC169500.011.12564856
CGATCTG151850.011.0301393
GCGACAT157500.011.0107287
GATCAAT185850.010.9356621
Produced by FastQC (version 0.11.5)