FastQCFastQC Report
Thu 12 May 2022
EGAF00006130845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142902363
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATAGATCGCTCGGA5046500.3531432156933612No Hit
CGGACTGGTCCAAGAG3319320.2322788742128778No Hit
TCTCAGCCATGCGTTA3235850.22643782314502384No Hit
CCTAAAGAGACACTTC3083690.21578999361962964No Hit
GTTCAAGTCTGAAAGA3026720.21180335555402957No Hit
GCTGTTCTCTACTTTG2988820.20915119507156085No Hit
ACCGCAGAGGCGTCCT2970140.20784400884959475No Hit
CACAACATCATCGCCT2951310.20652632595025738No Hit
GGTCATATCACATCCC2849800.199422874483888No Hit
AACCGATCAGGGTACA2791580.19534876410686086No Hit
TCACCACTCGCATACA2746340.1921829662116924No Hit
AGCCTTCAGGTTCTCA2745060.19209339456479105No Hit
ACAAGCTCAATGGTAA2742310.1919009554796515No Hit
ACAGGCCCACATCATG2610070.18264708470915908No Hit
TGTTAGGCAAGTAATG2605340.18231608948271905No Hit
AACGAGGTCGGTCAGC2572970.1800509065060037No Hit
GCAGATTAGAAATCTG2563500.17938821627463222No Hit
CCACGTTAGGCGACTA2546280.17818319771241292No Hit
GTTGGGCGTTCCAATG2517630.1761783323345045No Hit
TCAGTCCAGGTTAGTA2516120.1760726657823006No Hit
ATAGGCTCACGTTACA2495260.17461292784920568No Hit
TTGGTCCGTATCTCAG2463580.17239602958839806No Hit
TTACCCGAGCTACGTT2383590.16679850143555708No Hit
TCCGACTAGAACAGGA2351590.16455921026302414No Hit
ACCCAAAAGGCAGTGT2309810.1616355357258858No Hit
TATTGCTGTGTCCAGC2303050.1611624854656882No Hit
CCCTAGTTCACAGGGA2283510.15979511829346027No Hit
AAAGGATTCGGATGTT2257770.15799388845655407No Hit
CTCAACCAGTCGTATC2242090.1568966357820129No Hit
AAAGATGTCGGATAAA2215730.1550520196786389No Hit
AACTGGTTCGCGATGC2184670.15287850768429911No Hit
TTGCGAATCCACACCT2162000.15129210984425778No Hit
ATTGTCTAGGTCGTTT2151990.15059163157434982No Hit
ATTGTCTGTGGACTGA2109610.14762597032772648No Hit
TAGCATGAGTACCTCA2101880.14708504155386148No Hit
GGGACCTCACTTGCGG2084250.14585133207349413No Hit
TGGGTTACAATGCACT2081440.14565469431740607No Hit
ACTCGGGGTCATTGGT2075080.14520963519686514No Hit
ACTCGGGAGCACGTAG2071630.14496821161732645No Hit
CGTAAACCATCCGTAA2062090.14430062293651505No Hit
ACCGAATTCTCTATCA2050760.1435077739057401No Hit
AATACGCAGGTTCGAG2034660.14238113053455945No Hit
CCGTACTTCCACCTAC2031520.14216140008825467No Hit
GGACACCAGTACGCGA2026190.14178841815232965No Hit
CACCTTGAGTCGGGAT1995710.139655493310492No Hit
GAGCGCTTCCATAACG1990420.13928531048853265No Hit
AGCTGATCAGAATGCG1985420.13893542124282438No Hit
GCATGATAGATTACGA1980640.13860092712392727No Hit
GCAGCTGTCTTTGCAT1942400.13592497417275037No Hit
GTAATCGTCGCCACTT1933970.13533506090448624No Hit
ACTATTCCAGTAACTC1927960.1349144940311449No Hit
ATTGTCTGTGCGCATC1926380.13480392902950109No Hit
AAAGGATGTCTGGTTA1922940.13456320522845377No Hit
CATTCCGAGAAGGGCG1920490.13439175949805673No Hit
AGCTATGTCGGGAATG1916290.13409785253166176No Hit
GGACACCTCGGTCTAA1914950.13400408221381196No Hit
AGCTGTAAGACCAATA1900910.13302159321186313No Hit
GTTCAAGCATCCTCGT1898910.13288163751357981No Hit
TTGTTGTTCTCGTAGA1897760.1328011629870669No Hit
CACTGAACAGTAAGCG1895860.13266820507369775No Hit
ACTCGGGAGCCTTTGA1847350.12927357961183608No Hit
AACGGGATCTATCTTG1842170.12891109435328232No Hit
GCGGAAAAGGAGTACC1834890.12840165561153108No Hit
TACTAGGAGCCCATGC1828840.12797828962422406No Hit
TTGCGAACAGTAGGCA1817330.12717284458060363No Hit
ACGTTAGGTCGAGAAC1793210.1254849788593069No Hit
TCCATCGGTGTCCCAG1782680.12474811210784528No Hit
CTGGGACCAACTGCGC1765470.12354379332411738No Hit
GAGATTCGTTCCAATG1762590.12334225711858943No Hit
CTAGGATCACAAGGGT1747070.12225620089991093No Hit
AGGACGACAAGTGGCA1737200.12156551952888281No Hit
GAAGTGGTCAAGTTGC1724540.12067959995874947No Hit
CACCTGTAGTTATGAG1719590.12033320960549826No Hit
CAACGGCTCCTCCCAA1716470.12011487871617631No Hit
ACTCGGGGTAGCGAGT1715730.12006309510781148No Hit
TTGCAGATCAGGGTTT1714100.11994903121371057No Hit
GCGCCAACAACTCCCT1711520.1197684883629251No Hit
GCACCTTAGACTCTTG1710220.11967751715904096No Hit
GTAGACTCAATTGGCT1701090.11903861939637765No Hit
GATTGACCACCCATCT1684090.11784899596096951No Hit
TGGCCTTTCGCGGCAT1683410.11780141102355318No Hit
GCGGAAAGTTGGCTAT1664640.11648792679516433No Hit
TCGTTCGAGCGTATCT1659290.11611354530225648No Hit
TCGTTCGGTACCGGCT1655120.11582173767133577No Hit
GCCAGACTCGCACAAT1654160.1157545589361598No Hit
ACAGCGCTCCTGGAAT1651970.11560130744653957No Hit
AATGGCTAGGCAGTGT1650640.11550823690718118No Hit
GTAGTACAGGGAAATG1635940.11447956252479885No Hit
CCCTGATCAAGCAATA1635340.11443757581531383No Hit
AATACGCGTCTGGTCG1632410.1142325407173288No Hit
GCGGAAACATACAACC1625120.11372240219708614No Hit
CGGTGCATCGTGAGTT1624910.1137077068487664No Hit
AGGCGAAGTCTCGGGT1596950.11175112618676572No Hit
CAACGGCGTGAATACA1594580.11158527868429999No Hit
ATCCTCGAGGGAAATG1588380.11115141601962172No Hit
GGATGAGAGTCCCTCT1587740.11110663019617108No Hit
CCGTACTTCAATGCAC1586280.11100446253642425No Hit
CCTAAAGCACACACAT1585910.11097857073224184No Hit
CAGTGCGCACAGGAAC1565430.10954542438182076No Hit
TCCCACACATCATAGC1563150.10938587488577778No Hit
CTCTAGCTCACTCGGG1560410.10919413557912966No Hit
AACCTTTGTACGCAAG1559410.109124157729988No Hit
TTAGCGAGTTTCCGGG1553500.10871058864156082No Hit
GTAATCGGTAACTCCA1552290.10862591544409941No Hit
CCGTACTCATGGGAAC1552240.10862241655164233No Hit
GCGTAGCCAGTTAAAG1547080.10826133085007139No Hit
ATGTTTCAGAGGAATG1541590.1078771524582837No Hit
CCAGATAGTTGTGACT1535770.10746988137627926No Hit
TTTGGCCAGTCTGGGA1535370.10744189023662262No Hit
ATGTCTTTCCAGCACG1525730.10676730377089706No Hit
TCGGGACCAACTCGTA1525640.1067610057644743No Hit
GCGATTAAGTACAACA1521820.1064936903807532No Hit
CAGTATGAGAACAGGA1520380.10639292227798922No Hit
CTCTACGTCGGTTGTA1519480.10632994221376171No Hit
TCTATTGCAAGGAACC1515940.10608222062780026No Hit
AGCGTGCAGGCCAGTA1515130.10602553856999553No Hit
CAATCCCCAATTAGGA1511010.1057372298315319No Hit
TAGGTGTTCGTAGCGC1510740.10571833581226366No Hit
CTCTCGATCCATAACG1510310.10568824533713274No Hit
GCTCGAGTCCATAACG1499180.10490939187618611No Hit
CCACGTTAGAACGCCA1484830.10390520974100338No Hit
TTGCTTAGTATTGTCG1477650.10340276878416628No Hit
ATGCATGTCCCACTAC1474860.10320753058506107No Hit
GTGACATGTATTCTCT1473660.10312355716609108No Hit
TTGCCCAGTGGCGCTT1472170.10301929017087003No Hit
ATAGTCGCAGATAAGT1471980.1030059943795331No Hit
ATTCGTTAGACACTTC1471710.10298710036026487No Hit
TGCCTCATCTTCTCTC1470240.10288423292202664No Hit
CTTCCAGAGTCCAGAG1465330.10254064168274112No Hit
GCGTTGGCACTACACA1464440.10247836139700504No Hit
CCCACATGTAGGGTTG1460990.10223693781746632No Hit
GCCAGCATCTACCCGT1458610.1020703905365092No Hit
GAACTTGGTAGATGAT1452280.10162743075144251No Hit
ACAAAGAAGAGAAGCA1445170.10112988824404535No Hit
CCACAGGTCAACACGT1443270.1009969303306762No Hit
AGCCTTCAGTTCAGGG1438480.10066173643328766No Hit
GTAATCGTCGTGAACT1437180.10057076522940352No Hit
CTAGCGGTCATGCTTT1432760.1002614631361974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)