FastQCFastQC Report
Fri 31 Mar 2023
EGAF00006130995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006130995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15102700
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2843881.8830275381223223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1036020.685983300999159No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT673220.445761353930092No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359440.2379971793123084No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327360.2167559443013501No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTGACTGTGT177190.11732339250597576Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTGACTGT166740.11040409992915175Illumina Single End PCR Primer 1 (96% over 33bp)
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160220.10608699106782231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)