FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006131246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006131246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600012593
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG416050.014.33553694
ACCGCGC1138050.014.107641
CGCGATC806050.011.2594743
GCGCGAT793450.010.9466262
CCGCGCC1538550.010.4108412
CGCGCCC1217400.09.8061443
GGCGCGA911500.09.7764121
CTCACGC3087100.08.5836543
GCTCACG3155500.08.4244012
ACCGCAC1069800.07.78941
GCGATCT1595250.07.76335954
TAAGTCG279300.07.589329794
ACTGCGC1130650.06.8505721
ACGCGAT122500.06.82938772
CGCGCCT693600.06.69492533
CACGCCT4165900.06.6936555
CGCCCGG1892150.06.6321385
ACGCCTG4012200.06.62793456
GACGCGA151850.06.6236421
TCACGCC4198450.06.6081764
Produced by FastQC (version 0.11.5)