FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006131271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006131271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602488347
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG3423250.081.2479194
TAAGTCG2946500.042.75380794
GAAGTCG2066450.036.2771994
CAAGTCG1300800.034.1190894
AAAGTCG1344000.028.39933294
ATAAGTC3747600.011.45402494
CTAAGTC3375000.011.19365994
TTAAGTC4747550.08.68117294
GTAAGTC2361400.07.002032894
AGAAGTC5050000.06.49251594
GTCGGAT242700.06.27441394
TCGGATC294600.06.0305594
GGAAGTC3668400.05.861547594
ACAAGTC3028900.05.792565394
CGAAGTC559550.05.72853294
TGAAGTC5624050.05.38272394
Produced by FastQC (version 0.11.5)