FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006134035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795870
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC954105.31274535461921No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC653493.6388491371869907No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC232281.2934121066669637No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC165110.9193872607705458No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT43130.24016214982153497No Hit
GTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATT38730.21566148997421863No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC34510.19216312984792885No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG34250.1907153635842238No Hit
CCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATAT33680.18754141446763964No Hit
CCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTT33640.1873186811963004No Hit
GGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTG33430.18614933152176938No Hit
CCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCC33200.18486861521156878No Hit
CCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATC33190.18481293189373396No Hit
GAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACA33130.1844788319867251No Hit
GCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGG32330.1800241665599403No Hit
ATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTC31340.17451151809429413No Hit
GGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAG31290.17423310150512009No Hit
GATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTT31270.17412173486945046No Hit
GCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATA28210.15708263961199864No Hit
CCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGAC27950.15563487334829357No Hit
CTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACC27430.15273934082088347No Hit
GGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATA27240.15168135778202207No Hit
GTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGA26370.14683690913039363No Hit
CTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCC25700.14310612683546137No Hit
ATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAG25550.14227087706793923No Hit
ATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTC25430.1416026772539215No Hit
AATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATA25280.14076742748639937No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA24900.13865146140867657No Hit
CTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCT24700.13753779505198038No Hit
GTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGA23980.13352859616787408No Hit
GAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGTT23950.13336154621436963No Hit
GGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACAC22620.12595566494233992No Hit
GCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATATCTCCTTTT22570.12567724835316588No Hit
AGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGT21560.12005323325185008No Hit
AGAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACAT21500.11971913334484122No Hit
GATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAA21410.11921798348432794No Hit
ATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTT21160.11782590053845768No Hit
GAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATC20380.11348260174734251No Hit
CTCTAGAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTA20320.11314850184033366No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC20100.11192346884796785No Hit
CTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATC19850.1105313859020976No Hit
AGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATATCTCCT19410.10808131991736594No Hit
GACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCAT19280.10735743678551343No Hit
CTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGC19160.10668923697149571No Hit
GGTTGGTGCTGGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCT19100.10635513706448686No Hit
CCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAG18940.1054642039791299No Hit
CATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTC18880.10513010407212103No Hit
CGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAA18780.10457327089377294No Hit
AGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATG18680.10401643771542482No Hit
TTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTG18590.10351528785491156No Hit
GAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAA18490.10295845467656345No Hit
GTGCTGGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGA18210.10139932177718877No Hit
CAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGG17970.10006292214915333No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)