FastQCFastQC Report
Wed 8 May 2024
EGAF00006134057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211643
Total Bases182.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC661275.457630671740769No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC452703.7362490436539475No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC162901.3444554212750786No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC114620.9459882160009178No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT30280.24990859518851674No Hit
GTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATT25820.21309907291174052No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC24070.19865587470896956No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG23450.19353885591713071No Hit
CCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCC22710.18743144639138756No Hit
GAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACA22380.18470787187315074No Hit
GGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTG22290.18396507882272253No Hit
CCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTT21800.17992098332594667No Hit
GGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAG21790.17983845076478797No Hit
ATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTC21660.178765527469725No Hit
CCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATAT21640.17860046234740762No Hit
CCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATC21190.17488649709526652No Hit
GCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGG20900.17249305282166447No Hit
GATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTT20430.168614022447206No Hit
GCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATA19360.15978303840322602No Hit
CCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGAC18680.15417082424443504No Hit
CTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACC18320.15119965204272215No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA18080.14921887057491356No Hit
GGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATA18070.14913633801375487No Hit
AATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATA17990.14847607752448536No Hit
GTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGA17800.1469079588624702No Hit
CTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCC17130.1413782772648379No Hit
ATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTC16740.13815950737964897No Hit
ATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAG16660.13749924689037943No Hit
GAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGTT16620.13716911664574466No Hit
CTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCT16460.1358485956672056No Hit
GTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGA16100.13287742346549272No Hit
GGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACAC15390.12701761162322564No Hit
AGAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACAT15320.1264398836951148No Hit
GCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATATCTCCTTTT15220.12561455808352792No Hit
AGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGT14580.12033247416937166No Hit
GATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAA14370.11859929038503915No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC13950.11513292281637413No Hit
ATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTT13890.11463772744942198No Hit
GAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATC13560.11191415293118516No Hit
CTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATC13430.11084122963612218No Hit
CTCTAGAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTA13190.10886044816831361No Hit
GACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCAT13140.10844778536252014No Hit
AGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATATCTCCT13060.10778752487325062No Hit
GTGCTGGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGA12780.10547661316080727No Hit
CTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGC12730.10506395035501381No Hit
CGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAA12530.10341329913183998No Hit
AGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATG12460.10283557120372915No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG12410.10242290839793569No Hit
CCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAG12330.10176264790866615No Hit
AGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTTTC12290.10143251766403141No Hit
GAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAA12220.10085478973592056No Hit
CAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGG12170.1004421269301271No Hit
GGTTGGTGCTGGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCT12160.10035959436896842No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)