FastQCFastQC Report
Sat 28 May 2022
EGAF00006134065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345058
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC735905.471139534503345No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC506163.7631091001280246No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC183191.3619487040707539No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC125760.9349782685951089No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT32360.24058442089486104No Hit
GTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATT28220.20980507903748385No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC27630.2054186510916258No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG26150.1944154081087953No Hit
CCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCC25460.18928551779923247No Hit
CCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTT24960.18556820598070864No Hit
GAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACA24810.1844530124351515No Hit
CCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATAT24690.18356085759870577No Hit
GGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTG24610.18296608770774198No Hit
GCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGG23780.17679535008899244No Hit
CCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATC23680.17605188772528768No Hit
GGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAG23670.17597754148891723No Hit
ATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTC23460.17441627052513722No Hit
GATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTT22980.17084765117935435No Hit
GCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATA21100.15687055874170483No Hit
CTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACC20960.15582971143251814No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA20950.15575536519614766No Hit
GGGAGAAGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATA20680.15374801681414482No Hit
CCTGGGTCTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGAC20200.15017939746836195No Hit
GTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGA19640.14601600823161529No Hit
AATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATA19580.14556993081339245No Hit
ATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAG19550.145346892104281No Hit
CTTGTCGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCC19160.14244738888583242No Hit
ATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTC18710.13910180824916102No Hit
GTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGA18310.13612795879434197No Hit
CTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCT18180.13516145772152577No Hit
GAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGTT17920.13322845557589338No Hit
GGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGAGACAC17390.12928810504825816No Hit
GCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATATCTCCTTTT16770.1246786383932886No Hit
AGAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACAT16600.12341475237499053No Hit
GATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAA16390.12185348141121052No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC16310.12125871152024671No Hit
GTGCTGGCGGACTCCAGAATCAGGGAGAAGCGCTCCTTCTTCTCTCTAGA15860.11791313088357529No Hit
ATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTT15760.11716966851987051No Hit
AGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATCTCGAGCTCTGGGT15580.11583143626520194No Hit
CTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGC15270.11352670293771719No Hit
GAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATC14820.11018112230104576No Hit
GACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCAT14820.11018112230104576No Hit
CTCTAGAGACACTGTACCCCTCAGGAATATCTCCTTTTTCTTTCATTTTA14580.10839681262815432No Hit
AGCGCTCCTTCTTCTCTCTAGAGACACTGTACCCCTCAGGAATATCTCCT14210.10564600188244672No Hit
CTGGACACATTCCAGAAAAACTTTCTCTCCCGTCCTTTTGACTAGATATC14170.1053486169369648No Hit
GAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAA14100.10482819328237146No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG13990.10401038468229623No Hit
AGCCGTAGCCCCAGACCTGGGTCTTGTCGATACCAGAACATATTTTCATG13940.10363865350044385No Hit
CCTCAGGAATATCTCCTTTTTCTTTCATTTTAACATCATATGAGAAATAG13820.10274649866399814No Hit
TTTCATTTTAACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTG13700.10185434382755242No Hit
CGATACCAGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAA13640.10140826640932957No Hit
ACATCATATGAGAAATAGATCAGCCGTAGCCCCAGACCTGGGTCTTGTCG13540.10066480404562479No Hit
AGAACATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTTTC13470.10014438039103148No Hit
CATATTTTCATGGTCCATATCCTGGACACATTCCAGAAAAACTTTCTCTC13460.100070034154661No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)