FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006134323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30288296
Sequences flagged as poor quality0
Sequence length96
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16995495.6112400644790315Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2800490.9246112755897526Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1205260.39792928595256727Clontech SMART CDS Primer II A (100% over 25bp)
GTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTT1197120.39524177920078435No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG980470.3237124993759966No Hit
GGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGA656490.21674708937075893No Hit
CCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATAGG620240.20477876999089023No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT599210.1978354939478933Clontech SMART CDS Primer II A (100% over 23bp)
GCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTCCCCC588130.19417731522433615No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT539450.1781051003991773Clontech SMART CDS Primer II A (100% over 22bp)
GTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGAC489150.16149802550793876No Hit
GGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATTTTTG484620.16000239828612345No Hit
CCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCT468970.15483538591936635No Hit
CTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTA460830.15214787916758343No Hit
CCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTC446540.1474298851279055No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445710.14715585188417335Clontech SMART CDS Primer II A (100% over 21bp)
ACTGAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCA445050.14693794593132609No Hit
GATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATA418890.13830094634574358No Hit
AAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTT417660.13789484888816458No Hit
CCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCC398930.13171094207478692No Hit
GGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATTTTTGTTAATGTC375040.12382340690278515No Hit
CTGATGGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGA365710.12074300911480791No Hit
GGCTGCTGATGGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAAC361420.11932662042130068No Hit
GTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTA360000.11885779246214447No Hit
AAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTG355170.11726311707994401No Hit
GACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTT351390.1160151102590915No Hit
CCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATA349520.11539771005935759No Hit
GTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTCC333490.11010523668944598No Hit
ATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAAT320400.10578343529130856No Hit
GAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGA319830.10559524378657684No Hit
CGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTT317340.10477314405538034No Hit
CAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCT315820.10427130004276239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)