FastQCFastQC Report
Sat 28 May 2022
EGAF00006134407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35287047
Sequences flagged as poor quality0
Sequence length96
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20055035.683397083354694Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3437620.9741874971855821Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1491930.422798201277653No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1438330.4076084915804941Clontech SMART CDS Primer II A (100% over 25bp)
GCCGAATCTTGAGGGGACTCCAGTTTGCAAAGTGGAGCCATCATAGATCA627850.1779264782343504No Hit
CTCCAGTTTGCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGGCTCTC510910.14478683920476543No Hit
GGAGTTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACGACCCG484710.13736201841996015No Hit
GCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGGCTCTCCCTGGTTTC433650.12289211959277861No Hit
GTTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACGACCCGCGA421730.11951410952579851No Hit
GGGGACTCCAGTTTGCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGG410050.11620411308432808No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT385470.10923838427171308Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372240.10548913316549272Clontech SMART CDS Primer II A (100% over 22bp)
CTCAGATCCACTGCCGCCGAATCTTGAGGGGACTCCAGTTTGCAAAGTGG358560.10161235651144171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)