FastQCFastQC Report
Sat 28 May 2022
EGAF00006134409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23231425
Sequences flagged as poor quality0
Sequence length96
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13418305.775926358370182Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2284060.9831768821757597Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT971920.41836434915206455Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG784480.33768053401803805No Hit
GCCGAATCTTGAGGGGACTCCAGTTTGCAAAGTGGAGCCATCATAGATCA403200.17355801462889167No Hit
CTCCAGTTTGCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGGCTCTC332020.1429184821852297No Hit
GGAGTTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACGACCCG315930.13599251875423055No Hit
GCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGGCTCTCCCTGGTTTC283070.121847884923116No Hit
GTTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACGACCCGCGA270970.11663942267854857No Hit
GGGGACTCCAGTTTGCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGG268620.11562786182939705No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT257500.11084124198149703Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256180.11027304610027151Clontech SMART CDS Primer II A (100% over 22bp)
CTCAGATCCACTGCCGCCGAATCTTGAGGGGACTCCAGTTTGCAAAGTGG234770.10105708108736333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)