FastQCFastQC Report
Sun 5 Jun 2022
EGAF00006134553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36583917
Sequences flagged as poor quality0
Sequence length96
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20458825.5922989328890065Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3540560.9677913931414178Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1475460.4033083718181407Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1427860.39029718988264706No Hit
GCCGAATCTTGAGGGGACTCCAGTTTGCAAAGTGGAGCCATCATAGATCA652640.17839533147858389No Hit
CTCCAGTTTGCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGGCTCTC528600.1444897220819739No Hit
GGAGTTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACGACCCG496180.13562790446960615No Hit
GCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGGCTCTCCCTGGTTTC449280.12280806344492855No Hit
GTTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACGACCCGCGA430910.11778673125679791No Hit
GGGGACTCCAGTTTGCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGG427100.11674529001364178No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT395790.10818688441699667Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389260.10640194706324094Clontech SMART CDS Primer II A (100% over 22bp)
CTCAGATCCACTGCCGCCGAATCTTGAGGGGACTCCAGTTTGCAAAGTGG368580.10074918986941722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)