FastQCFastQC Report
Sat 28 May 2022
EGAF00006134737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46428858
Sequences flagged as poor quality0
Sequence length96
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25634015.521137306457118Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4385510.944565554466147Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1956360.42136724534555636No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1840400.3963913995041618Clontech SMART CDS Primer II A (100% over 25bp)
GCCGAATCTTGAGGGGACTCCAGTTTGCAAAGTGGAGCCATCATAGATCA845340.1820721069641644No Hit
CTCCAGTTTGCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGGCTCTC685210.14758278138135553No Hit
GGAGTTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACGACCCG638140.13744469011062044No Hit
GCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGGCTCTCCCTGGTTTC573450.1235115453410463No Hit
GTTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAGGCCAACGACCCGCGA551540.11879249754538437No Hit
GGGGACTCCAGTTTGCAAAGTGGAGCCATCATAGATCAGGAGTTTAGGGG548640.11816788601606354No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT489580.10544734914651573Clontech SMART CDS Primer II A (100% over 23bp)
CTCAGATCCACTGCCGCCGAATCTTGAGGGGACTCCAGTTTGCAAAGTGG480480.10348736124416415No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470430.10132275922013848Clontech SMART CDS Primer II A (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)