FastQCFastQC Report
Sat 28 May 2022
EGAF00006134753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29990055
Sequences flagged as poor quality0
Sequence length96
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16767355.5909700732459475Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2767690.9228692644945132Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1190460.39695158945190334Clontech SMART CDS Primer II A (100% over 25bp)
GTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTT1178080.3928235543415976No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG976950.3257579887732784No Hit
GGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGA644800.2150046073606734No Hit
CCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATAGG611320.20384090659386922No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT590810.1970019728206567Clontech SMART CDS Primer II A (100% over 23bp)
GCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTCCCCC583950.19471454787261977No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532330.17750217530444676Clontech SMART CDS Primer II A (100% over 22bp)
GTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGAC487440.16253387998121377No Hit
GGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATTTTTG475680.1586125800702933No Hit
CCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCT454830.1516602753812889No Hit
CTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTA452440.1508633445320457No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442700.14761560123847722Clontech SMART CDS Primer II A (100% over 21bp)
CCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTC442240.14746221705828816No Hit
ACTGAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCA440620.14692203798892667No Hit
GATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATA413950.1380290899766606No Hit
AAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTT408770.13630185073018372No Hit
CCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCC394760.13163030211181673No Hit
GGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATTTTTGTTAATGTC366900.12234055589427895No Hit
CTGATGGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGA363210.12111014801406667No Hit
GTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTA357000.11903946158151428No Hit
GGCTGCTGATGGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAAC356710.11894276285922116No Hit
AAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTG354010.11804246441028535No Hit
GACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTT349280.11646527490529777No Hit
CCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATA346700.1156049897207591No Hit
GTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTCC330740.11028322555593846No Hit
GAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGA316380.1054949715830798No Hit
ATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAAT315960.10535492515768978No Hit
CAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCT310490.10353098718891979No Hit
CGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTT307690.10259734435298636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)