FastQCFastQC Report
Sat 28 May 2022
EGAF00006134759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006134759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26741141
Sequences flagged as poor quality0
Sequence length96
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15378535.750887742598568Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2536970.9487141928611048Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1097120.4102741913667783Clontech SMART CDS Primer II A (100% over 25bp)
GTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTT1028840.38474050153656497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG904720.33832512980654045No Hit
GGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGA566830.2119692648866404No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT535780.20035794284170597Clontech SMART CDS Primer II A (100% over 23bp)
CCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATAGG530070.19822265624342655No Hit
GCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTCCCCC507790.18989092499830132No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT486890.1820752525107287Clontech SMART CDS Primer II A (100% over 22bp)
GTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGAC431530.16137306930919665No Hit
GGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATTTTTG426950.15966035256311614No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402530.15052835628816288Clontech SMART CDS Primer II A (100% over 21bp)
CCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCT399140.14926064673156617No Hit
CTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTA398340.1489614822344342No Hit
CCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTC384570.14381211332755023No Hit
ACTGAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCA380690.1423611655164602No Hit
GATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATA366440.13703229791129706No Hit
AAAATCTCCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTT360040.13463898193424134No Hit
CCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCC352900.13196893879733854No Hit
GGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATTTTTGTTAATGTC327500.1224704660133986No Hit
AAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTG325220.1216178471965725No Hit
CTGATGGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAACCTTGA315720.11806526879313041No Hit
GGCTGCTGATGGTGAAAGTAAAATCTCCCCCAGATCCACTTCCACTGAAC314240.11751181447343627No Hit
GTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTA312210.11675268456196392No Hit
GACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTT307690.1150624051531683No Hit
CCCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCTTTTTCCAAATA297510.11125553692716403No Hit
GTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTCC280650.10495064515010785No Hit
CAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCT278610.10418777568242132No Hit
ATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAAT278130.10400827698414214No Hit
GAACCTTGATGGGACCCCTTTTTCCAAATAGGATGCATCGTAGATCAGGA276490.10339498976502162No Hit
CGTAGATCAGGAGCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTT271160.1014018063028799No Hit
CTTTTTCCAAATAGGATGCATCGTAGATCAGGAGCTTAGGGGCTTTCCCT268280.1003248141132048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)